[Objective] The objective of this study is to investigate the effects of various tillage operations on slope shape and micro-topographic features on typical sloping cropland slopes of southwestern alpine-canyon area. [Methods] 50-time conventional tillage and contour tillage events were applied to rectangular plots (5 m × 20 m) with a slope of 15° to examine the impacts of tillage disturbance on the slope shape and micro-topographic features by combining the stylus method and 3D laser scanning technology. [Results] After 50-time conventional tillage, the slope shape gradually evolved from a linear slope to a “”-type composite slope, while contour tillage evolved to a “”-type . After 50- time successive tillage, the range of soil loss under conventional tillage and contour tillage accounted for 15% and 11%, and the range of accumulated soil accounted for 13% and 6% on the whole slope, respectively. The two kinds of tillage disturbance patterns caused significant changes in the surface roughness and topographic undulation on the slope, and the degree of change of conventional tillage was greater than that of contour tillage. The average thickness of the soil loss by conventional tillage and contour tillage were 1.17 and 0.45 cm/time, respectively. The indirect effect of tillage disturbance patterns on soil loss was the largest, while the direct effect of tillage frequency on soil loss was the largest. [Conclusion] Tillage frequency and tillage pattern were the most important factors affecting slope shape and micro-topographic features. As the frequency of tillage increased, and the amount of soil loss gradually increased.