Forest fire, as the main natural and human disturbance process of subtropical forest ecosystem, seriously affects the soil biochemical cycle. However, the response of soil extracellular enzyme activities to the restoration process after forest fire disturbance is still unclear. In this study, the soils of subtropical secondary forests at different restoration stages after forest fire disturbance were selected as the research object, and the subtropical forest soils in seven restoration stages of secondary forest were selected by using the method of spatial substitution time series, and the soil was divided into old forest (60-70 years), middle-aged forest (30-40 years) and young forest (<20 years). Through the determination of soil carbon (C), nitrogen (N), phosphorus (P) related invertase activities, to reveal the forest fire disturbance of subtropical forest soil extracellular enzyme activity and its stoichiometric characteristics succession rule. The results showed that the activities of soil cellulose hydrolase (CBH) and β-N-acetylglucosaminidase (NAG) increased significantly with the time of succession, while the activities of other enzymes did not change significantly. The results of correlation analysis and redundancy analysis showed that soil C and N contents were the key factors affecting the change of soil extracellular enzyme activity. In addition, the ratios of soil extracellular enzyme C, N and P in young, middle and old forests were 1:0.94:1.53, 1:1.02:1.63 and 1:0.99:1.47, respectively, indicating that the growth of forest soil microorganisms in subtropical areas was limited by P, and improves gradually with the increase of restoration years after forest fire disturbance. In summary, after forest fire disturbance, the extracellular enzyme activity of forest soil showed an increasing trend with the restoration time, and the stoichiometric characteristics of soil extracellular enzyme gradually tended to be stable, indicating that soil health status of subtropical forest was gradually improved with the restoration process of forest fire disturbance.