[Objective] To explore the transpiration characteristics of common trees and their response to meteorological factors in karst slope secondary forest. [Methods] Heat diffusion probe method (TDP) was used to monitor Broussonetia papyrifera, Koelreuteria paniculata, Triadica sebifera, Ailanthus altissima, Melia azedarach, Toona sinensis, and Rhus chinensis in Puding Karst Ecosystem Observation and Research Station of Chinese Academy of Sciences, and to study the relationship between tree transpiration characteristics and meteorological factors. [Results] (1) The average daily transpiration of Broussonetia papyrifera, Koelreuteria paniculata, Triadica sebifera, Ailanthus altissima, Melia azedarach, Toona sinensis, and Rhus chinensis were: (4 409.97±3 260.63) g/d, (6 611.27±5 629.57) g/d (3 956.32±2498.50) g/d (2984.98±2039.09) g/d (6 300.25±4 637.71) g/d, (780.69±738.80) g/d, (2320.73)±1717.18) g/d.(2) The fluid flow rates of Broussonetia papyrifera, Koelreuteria paniculata, Triadica sebifera, Ailanthus altissima, Melia azedarach, Toona sinensis, and Rhus chinensis were the highest in sunny days, followed by cloudy days, and the lowest in rainy days. (3) The influence of atmospheric temperature (T) and saturation water vapor pressure difference (VPD) on Broussonetia papyrifera, Koelreuteria paniculata, Triadica sebifera, Ailanthus altissima, Melia azedarach, Toona sinensis, and Rhus chinensis under sunny weather was the greatest, followed by relative humidity (RH) and total irradiance (Eg). [Conclusion] The transpiration of common trees in secondary forest of karst slope is limited by special water environment. Different deciduous trees have different water use strategies, and the liquid flow rate is mainly affected by VPD and Eg.