外源碳酸钙和温度对黄壤活性有机碳组分及微生物群落组成的影响
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赵娜(1998-),女,在读硕士研究生,主要从事土壤有机碳稳定性研究。E-mail:3082054232@qq.com

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国家自然科学基金项目(31860160)


Effects of Exogenous Calcium Carbonate and Temperature on Active Organic Carbon Components and Microbial Mommunity Composition in Yellow Soil
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    摘要:

    为探究不同温度条件下添加碳酸钙对活性有机碳组分及微生物群落组成的影响,以贵州典型黄壤土为研究对象,采用同位素标记法,设置6个处理(培养温度分别为15,25,35 ℃下添加碳酸钙和不添加碳酸钙),通过分析不同温度下碳酸钙添加对土壤活性有机碳、微生物群落组成的影响,揭示外源碳在土壤活性碳库中的分配规律,以期为贵州典型黄壤有机碳固存和改良提供理论依据。结果表明:各温度下添加碳酸钙显著增加土壤DOC和MBC含量(p<0.01),添加碳酸钙各处理土壤DOC含量在培养第5天均达到最大值,相较于不添加碳酸钙处理,添加碳酸钙处理土壤DOC含量在15,25,35 ℃下分别显著提高83.41%,80.37%,90.41%;添加碳酸钙处理温度对土壤DOC、MBC具有显著影响(p<0.05),在培养的第15,60天,土壤DOC和MBC含量均在不同温度下达到显著性差异,土壤DOC含量大小依次为35,15,25 ℃,土壤MBC含量多少依次为15,25,35 ℃。同位素标记发现,在培养第1和第5天,13C-DOC,13C-MBC含量在15,25,35 ℃下达到峰值,且13C-DOC对土壤总DOC贡献率分别为16.85%,21.20%和15.22%,13C-MBC对土壤总MBC的贡献率分别11.95%,10.49%和17.18%。添加碳酸钙各处理均显著提高土壤pH。高通量测序发现,在培养第60天,外源碳酸钙输入对细菌群落组成相对丰度及群落结构具有显著影响,但对真菌群落组成影响较小。添加碳酸钙处理提高变形菌门相对丰度,降低绿弯菌门相对丰度。相关分析表明,土壤pH与优势菌门变形菌门(Proteobacteria)、担子菌门(Basidiomycota)呈显著正相关,与绿弯菌门(Chloroflexi)、子囊菌门(Ascomycota)呈显著负相关;绿弯菌门与土壤DOC呈显著负相关,放线菌门与土壤MBC、13C-DOC及担子菌门与DOC、13C-MBC、13C-DOC呈显著正相关。综上表明,外源碳酸钙对土壤活性有机碳的影响大于温度,外源碳酸钙增加土壤pH、DOC和MBC含量,改变土壤微生物群落组成丰度。因此,碳酸钙添加是黄壤有机碳固存和改良的有效措施。

    Abstract:

    In order to explore the effects of adding calcium carbonate on active organic carbon components and microbial community composition under different temperature conditions, the typical yellow soil in Guizhou province was taken as the research object, and six treatments were set up (the treatments with and without adding calcium carbonate at culture temperatures of 15, 25, 35 ℃) by isotope labeling method. By analyzing the effects of calcium carbonate addition on soil active organic carbon and microbial community composition at different temperatures, the distribution rule of foreign carbon in soil active carbon pool was revealed, with a view to providing a theoretical basis for organic carbon sequestration and improvement of typical yellow soil in Guizhou. The results showed that: The addition of calcium carbonate significantly increased the contents of DOC and MBC in soil at different temperatures (p<0.01), and the contents of DOC in soil treated with calcium carbonate reached the maximum value on the 5th day of culture. Compared with the treatment without calcium carbonate, the soil DOC content in the treatment with calcium carbonate were significantly increased by 83.41%, 80.37% and 90.41% at 15, 25 and 35 ℃, respectively. The temperature of calcium carbonate addition had significant effects on soil DOC and MBC (p<0.05). On the 15th and 60th day of culture, the contents of DOC and MBC in soil showed significant differences at different temperatures, and the overall content of DOC in soil was 35 ℃>15 ℃>25 ℃, and the overall content of MBC was 15 ℃>25 ℃>35 ℃. Isotopic labeling showed that the contents of 13C-DOC and 13C-MBC peaked at 15, 25 and 35 ℃ on the 1st and 5th day of culture, and the contribution rate of 13C-DOC to the total soil DOC was 16.85%, 21.20% and 15.22%, respectively. The contribution rate of 13C-MBC to total soil MBC was 11.95%, 10.49% and 17.18%, respectively. The addition of calcium carbonate significantly increased soil pH. High-throughput sequencing showed that the input of exogenous calcium carbonate had significant effects on the relative abundance and community structure of bacterial community composition, but had little effect on fungal community composition at day 60 of culture. The addition of calcium carbonate increased the relative abundance of Proteobacteria and decreased the relative abundance of Chlorocurvula. Correlation analysis showed that soil pH was positively correlated with the dominant bacteria Proteobacteria and Basidiomycota, and negatively correlated with Chloroflexi and Ascomycota. The chloromycetes was significantly negatively correlated with soil DOC, while the actinomycetes was significantly positively correlated with soil MBC, 13C-DOC and basidiomycetes was significantly positively correlated with DOC, 13C-MBC and 13C-DOC. In conclusion, the effect of exogenous calcium carbonate on soil active organic carbon was greater than that of temperature. Exogenous calcium carbonate increased soil pH, soil DOC and MBC contents, and changed the composition and abundance of soil microbial community. Therefore, calcium carbonate addition is an effective measure for sequestration and improvement of organic carbon in yellow soil.

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赵娜, 李瑞东, 王小利, 段建军, 郑钦文, 李明瑞.外源碳酸钙和温度对黄壤活性有机碳组分及微生物群落组成的影响[J].水土保持学报,2023,37(6):238~245

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  • 收稿日期:2023-05-23
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  • 在线发布日期: 2023-12-27
  • 出版日期: 2023-12-28