In order to explore the effects of bio-organic fertilizer combined with reduced fertilizer on nutrient utilization and yield of continuous cropping cucumber, a field plot experiment with nine treatments (T1~T9) was conducted, and cucumber was taken as the test crop. The nine treatments (T1~T9) were divided into three treatment groups, which were CK treatment group with 0 t/hm² bio-organic fertilizer addition (T1 reduced fertilizer 0%, T2 reduced fertilizer 10% and T3 reduced fertilizer 20%), Y1 treatment group with 10 t/hm² bio-organic fertilizer addition (T4 reduced fertilizer 0%, T5 reduced fertilizer 10% and T6 reduced fertilizer 20%) and Y2 treatment group with 20 t/hm² bio-organic fertilizer addition (T7 reduced fertilizer 0%, T8 reduced fertilizer 10% and T9 reduced fertilizer 20%). The results showed that compared with CK treatment, the cucumber yield of Y1 and Y2 treatment groups increased by 1.48%~38.88% and 15.31%~50.91%, respectively, the nitrogen utilization ratio of Y1 and Y2 treatment groups increased by 1.51%~10.07% and 6.41%~18.71%, the phosphorus utilization ratio of Y1 and Y2 treatment groups increased by 2.07%~5.38% and 5.67%~8.90%, and the potassium utilization ratio of Y1 and Y2 treatment groups increased by 9.50%~16.31% and 16.95%~28.43%, respectively. Under the condition of applying the same amount of bio-organic fertilizer, with the decreasing of chemical fertilizer application, the cucumber yield in CK treatments group (T1, T2 and T3) was significantly different. In Y1 group, the cucumber yield of T4 and T5 was significantly different from that of T6. In Y2 group, the cucumber yield of T8 was significantly different from that of T9. Among the nine treatments, T8 had the highest yield of cucumber, while the utilization rate of nitrogen, phosphorus and potassium of each treatment in Y1 (T4, T5 and T6) and Y2 group (T7, T8 and T9) all showed increase trends. The results could provide scientific basis for improving nutrient utilization rate and reducing crop continuous cropping obstacles.