In order to explore the effect of different sediment concentrations on infiltration characteristics of muddy water in layered soil, using the indoor free infiltration test of film hole irrigation, the cumulative infiltration, the wetting front migration law and the distribution of soil water in the wet body of clear water and different sediment concentrations (2%, 5%, 7% and 9%) of muddy water in layered soil with 5-10 cm sand layer were studied. The relationships of the cumulative infiltration volume, the wetting front migration distance and the infiltration time were established. The cumulative infiltration volume model of per unit film hole area under different muddy water sediment concentrations was proposed based on clear water infiltration. The results showed that there was a consistent trend for the cumulative infiltration under different sediment concentrations. The larger the sediment concentration was, the smaller the cumulative infiltration was, resulting in the more obvious of infiltration reduction force with muddy water. The smaller the infiltration coefficient was, the larger the infiltration index was. The wetting front migration distance decreased with the increasing of sediment concentrations. When the water infiltrated into the loam-sand interface, the wetting front had a significant stagnation in the vertical direction, which advanced with the infiltration time only in the horizontal direction. The soil moisture in the wet body mainly concentrated above the sand layer, and the soil water contents decreased with the increase of the muddy water sediment concentrations. These results could provide a theoretical reference for further research of muddy water film hole irrigation technology in layered soil.