The content of available nitrogen (N) is generally low in coal mining subsidence reclamation soil. Appling organic and inorganic combined fertilizers can improve soil N fertility, whereas N availability is unclear after fertilization, especially in a new coal mining reclaimed soil. This experiment was conducted in coal subsidence reclamation soil of Shuiyu Coal Mining, which located in Xiaoyi City, Shanxi Province. The experiment included eight treatments which were three organic-inorganic combined treatments (application rate of chicken manure and chemical fertilizer was 1:1, and N at 100, 150 and 200 kg/hm², expressed with MF100, MF150 and MF200), four single application of different amounts of chemical fertilizer nitrogen (N at 0, 100, 150 and 200 kg/hm², expressed with IF0, IF100, IF150 and IF200), as well as control (no fertilization, CK). We evaluated corn yield, aboveground nitrogen uptake, nitrogen use efficiency and mineral nitrogen content in soil profile after crop harvest, so that the suitable fertilization treatment and optimum nitrogen application rate were determined, which could provide scientific theoretical basis for reclaiming mining area soil in a way of high-yield and high-efficiency fertilization. The results showed that:(1) Under the condition of 150 kg/hm² nitrogen fertilizer, the grain yield of MF150 was 12.45% higher than that of IF150 (P<0.05). Furthermore, there was no significant difference in grain yield among IF150, IF200 and MF200. (2) Nitrogen uptake of corn aboveground increased with the increasing of nitrogen application rate, while at the same nitrogen rate, the combined application treatment significantly increased nitrogen uptake by 39.45%~41.46% relative to single chemical fertilizer treatment (P<0.05). (3) At the same nitrogen rate, combined fertilizer treatment could significantly improve nitrogen recovery rate compared with single chemical fertilizer application. The partial productivity of nitrogen fertilizer in differentfertilization patterns showed a downward trend with the increasing of nitrogen application rate. The highest nitrogen agronomic efficiency (24.08 kg/kg) was observed in the IF100 treatment, and there was no significant difference between MF150 and IF100. However, at the same nitrogen rate, agronomic nitrogen efficiency of MF150 significantly increased by 49.56% compared with IF150. (4) After harvesting, mineral nitrogen content in 0-40 cm soil profile increased with soil depth, whereas at the same nitrogen rate there was no significant difference among different treatments. At 40-60 cm depth, the residual mineral nitrogen content of single chemical fertilizer application increased by about 18% compared with the combined fertilizer treatment. In a word, MF150 not only improved grain yield, nitrogen uptake and nitrogen use efficiency, but also reduced residual mineral nitrogen content in soil profile after plant harvest, hence MF150 could be used as a recommended fertilization treatment for this coal mining subsidence reclaimed soil or low-yield farmland similar to the soil type in this experiment.