Astragalus membranaceus could accumulate selenium (Se) generally, but the uptake and translocation Se in this plant are still not clear. The soil pot experiment was conducted to explore the effects of different concentrations (0, 2, 4, 6, 8 mg/kg soil) as sodium selenite (SeIV) and sodium selenate (SeVI) added in soil on Se uptake, translocation and accumulation in Astragalus membranaceus. The results showed that the application of Se at the rate of 2～6 mg/kg as SeIV increased the shoots and roots dry weights of Astragalus membranaceus by 34.55%～38.24%,, compared to the control, whereas the application of Se with more than 2 mg/kg as SeVI inhibited the growth of Astragalus membranaceus. Moreover, the application of different concentrations Se as SeVI and SeIV increased Se concentrations in the shoots and roots, and there were the significant and positive relationships between Se concentrations added in soil and that in plants (SeVI: r=0.884**; SeIV: r=0.973**). With the increasing of Se application rates, the Se uptake capacity of Astragalus membranaceus was higher than the control, and the bioaccumulation factors and transfer factors were more than 1, and Se concentrations in plant shoots were higher than those of roots, which indicated that exogenous Se might improve the uptake and transport of selenium in Astragalus membranaceus. In conclusion, sodium of selenite and selenate added in the soil with pH＞7.0 could enhanced the uptake, transport and accumulation of Se in Astragalus membranaceus, and the application of 2～6 mg/kg in soil would be better for the growth of this plant and Se accumulation in the shoots and roots.