In order to effectively prevent and control the soil and water loss and pollution in Camellia oleifera field, by using the location observation of runoff plot under natural rainfall conditions, the effects of intercropping with Lolium perenne L. and Vulpia myuros C. Gmelin on soil nutrient loss in C. oleifera field were studied. The results showed that: (1) The intercropping of L. perenne and V. myuros with C. oleifera could improve soil water content and surface coverage, reduce soil bulk density, and increase soil fertility significantly through improving soil organic matter and nutrient contents in topsoil. (2) During April-July, the intercropping of L. perenne and V. myuros with C. oleifera could reduce surface runoff by 18.5%～58.9% and 17.9%～70.3%, respectively. (3) The reducing effect on surface runoff nutrient loss was best under intercropping with V. myuros, the total loss of total nitrogen, total soluble nitrogen, total phosphorus, total soluble phosphorus, total potassium and total soluble potassium decreased by 62.48%, 56.17%, 59.72%, 57.97%, 62.97% and 61.76%, respectively, compared with the control. The reducing effect on nutrient loss was least under the intercropping with L. perenne, but the reduced rates were even 30.95%, 24.42%, 25.03%, 28.22%, 32.97% and 30.42%, respectively for the indexes mentioned above. The nutrient concentrations of nitrogen, phosphorus and potassium in different forms were lower than those of the control. These findings showed that the effect of intercropping with green manures on soil and water conservation was remarkable in C. oleifera field, especially with V. myuros.