Pot experiments with selenium-enriched soil were conducted to investigate the effects of moisture condition on selenium forms in soil and selenium accumulation in rice. The treatments included alternation of wetting and moderate drying (AWMD), alternation of wetting and severe drying (AWSD), conventional (C) and flooding (F) water management. The results showed that the rice grain yield was the highest in the AWMD treatment, 22.17% higher than that of the F treatment, and the difference between these two treatments was significant. The selenium contents in different parts of rice were in the order of root (0.411 ~ 0.695 mg/kg) > stem (0.214 ~ 0.378 mg/kg) > leaf (0.187 ~ 0.327 mg/kg) > shell (0.156 ~ 0.235 mg/kg) > grain (0.132 ~ 0.185 mg/kg) in all treatments. The selenium contents of grain and available selenium contents in soil both were the highest in the AWSD treatment, and the lowest in the F treatment，and the selenium contents of grain was significantly different between the two treatments. Significant or extremely significant positive correlations were found between the available selenium contents in soil and selenium contents in roots, stem, shell and grain of rice. The correlation coefficients were 0.939**, 0.896**, 0.793** and 0.723*, respectively. The selenium absorption coefficient in rice root was the highest in the AWSD treatment (0.438), and the lowest in F treatment (0.259), indicating that the dry and wet alternation enhanced selenium absorption capacity of rice. In summary, wetting and drying alternated irrigation could not only improve rice yield, but also increase selenium absorption of rice, and this kind of irrigation saved water than normal management. The overall performance of the AWMD treatment was the best.