Apple replant disease (ARD) is a major factor that restricted the sustainable development of apple industry. In this study, the apple plant seedling biomass, root repiration rates, soil enzyme activities and soil environments under different phosphorus levels in continues cropping soil were determined to reveal the effect of biochar combined with phosphorus fertilizer on the control of ARD and select suitable amount of phosphate fertilizer, so as to provide theoretical basis for the renewal of old orchard and the prevention and control of ARD. Pot experiments including six treatments, which were the control (CK), methyl bromide sterilization (F), 2% biochar (B ), 2% biochar + 0.5‰ phosphate fertilizer (BP0.5), 2% biochar + 1‰ phosphate fertilizer (BP1) and 2% biochar + 2‰ phosphate fertilizer (BP2), were carried out. The biomass and root respiration rate of Malus hupehensis Rehd. seedlings, soil nutrient and soil enzyme activity were measured by conventional methods. The results showed that methyl bromide sterilization treatment increased heights, ground diameters as well as fresh and dry weights of seedlings, which were 1.6, 1.5, 2.9 and 2.5 times of the seedlings in the control, respectively. The root respiratory rates and activities of protective enzymes in the F treatment were all higher than those in the control or other treatments. The treatments of B and BP0.5 both significantly enhanced heights, ground diameters, fresh and dry weights of seedlings, which were 1.2, 1.1, 1.6, 1.4 and 1.4, 1.2, 2.2 and 1.9 times of the seedlings in the control, respectively. The root respiration rates increased significantly in the treatments of B and BP 0.5. Biochar combined with 0.5‰ phosphate fertilizer improved the activities of SOD, POD and CAT, and decreased the content of MDA in the seedling roots. Compared with the control, biochar combined with 0.5‰ phosphate fertilizer significantly improved the content of organic matter in soil, increased the contents of nitrate nitrogen and available phosphorus, and promoted the activities of urease, sucrase and phosphatase in soil. In conclusion, compared to the single application of biochar, application biochar combined with phosphate fertilizer could improve the development of M. hupehensis seedlings under continues cropping, increase soil organic matter content, enhance soil enzyme activity. Therefore, the integrated measures of biochar and phosphate fertilizer can better prevent and control ARD.