The effects of different cutting roots and fertilization measure on soil nutrient,microbe quantity and enzyme activity both rhizosphere and non-rhizosphere soil in I-107 poplar plantation were studied by field plot experiment.Results showed that the pH value in rhizosphere soil of same treatments poplar plantation was lower than that in non-rhizosphere soil,but the content of organic,available nitrogen,available phosphorus,available K,utility cuprum and utility zincum were all higher than those in non-rhizosphere soil.The available nutrient content of cutting roots fertilizer treatment was significant more than other treatments both in rhizosphere and non-rhizosphere soil.Cutting roots fertilizer treatment increased the activity of soil urease,invertase,catalase,polyphenol oxidase,peroxidase significantly,compared with CK.Five soil enzyme activities by fertilizer treatment increased little,but cutting roots treatment decreased the activity.The quantities of microbe were orderly followed as cutting roots fertilizer,fertilizer,cutting roots,CK.The results implied that the measure of cutting roots fertilization may improve the root soil environment and enhance the plantation soil fertility.