Using the remote sensing images and meteorological data of the Yunnan-Guizhou Plateau in 2000,2010 and 2018 and combining with the calculation method of ecosystem resilience,the ecosystem resilience of the Yunnan-Guizhou Plateau was calculated,the spatial and temporal distribution characteristics of the ecosystem resilience in 25 cities of the Yunnan-Guizhou Plateau were analyzed.,and the main influencing factors of its changes were also explored.The results showed that the ecosystem resilience of the Yunnan-Guizhou Plateau increased steadily from 2000 to 2018,indicating that the self-regulation and anti-disturbance capabilities of the ecosystem in this region were continuously enhanced.Spatially,the distribution of ecosystem resilience in the Yunnan-Guizhou Plateau was high in the southwest,low in the northwest,relatively lower in the middle,and relatively higher in the eastern.The ecosystem resilience was divided into four grades,among which the proportion of weak and medium grades showed a decreasing trend;the proportion of strong and very strong grades increased year by year.The driving factor analysis showed that rainfall and temperature were the main factors affecting the spatial distribution of ecosystem resilience.The results can provide a certain scientific basis for ecosystem protection in the Yunnan-Guizhou Plateau.