This study aimed to explore the effects of bio-organic fertilizer application on soil moisture, salinity, and soil nutrient properties in jujube fields under different irrigation levels, providing suitable water and fertilizer solutions for jujube cultivation. Two irrigation levels (W1 6 000 m³/hm², W2 6 600 m³/hm²), three basal application levels (S1-S2-S3: 1 200—1 650—2 100 kg/hm²) were set up with no bio-organic fertilizer as the control (W1CK, W2CK), totally eight treatments. A field experiment was conducted. Applying bio-organic fertilizer could increase soil water content, soil organic matter, and total carbon content at the same irrigation level. The increase in fertilizer application all showed that S3 > S2 > S1 > CK. At the W1 irrigation level, the water content, soil organic matter, and total carbon content of 0—150 cm soil increased by 3.20%~17.97%, 9.96%~20.31% and 2.03%~5.42% after the application of bio-organic fertilizer compared with the CK. At the W2 irrigation level, the above indices increased by 5.37%~20.19%, 6.55%~21.18% and 2.09%~6.34%, respectively. Application of bio-organic fertilizer could reduce the salinity content and soil pH of 0—150 cm soil at the same irrigation level. The increase in fertilizer application all showed that S3 < S2 < S1 < CK. At the W1 irrigation level, the salinity content and soil pH of the 0—150 cm soil decreased by 7.70%~15.74% and 0.86%~2.87% after the application of bio-organic fertilizer compared with the CK, while both indices were reduced by 8.90%~23.66% and 0.96%~2.52% respectively at the W2 irrigation level. In the comparison of different irrigation and fertlization (bio-organic fertilizer) treatments, the W1S3 treatment increased soil water content, soil organic matter, and total carbon content by 4.45%, 21.85% and 7.31%, respectively, compared with the local conventional irrigation treatment (W2CK) at 0—150 cm, soil salinity content and soil pH decreased by 2.59% and 2.08%, respectively. Considering the local water scarcity and easy loss of water fertilizer, and also the risk of groundwater pollution reduction, it is suggested that W1S3 treatment instead of the local conventional irrigation treatment (W2CK) should be a more appropriate irrigation and fertilization management.