In order to investigate the variation law and main driving factors of baseflow in the blown-sand region of Wuding River Basin, this study was based on the hydrological data of Zhaoshiyao hydrological station and precipitation data of several stations in the blown-sand region of Wuding River Basin from 1954 to 2018, compared the applicability of the three kinds of methods (HYSEP, Digital Filtering and BFI method) to segmentation baseflow, revealed the variation characteristics of river baseflow, analyzed the driving factors (precipitation, vegetation coverage and agricultural irrigation, etc.) which can cause changes of baseflow in this area. The results showed that the local minimum method in the HYSEP method is better than the other methods for the segmentation of channel basic flow in the blown-sand region. The average basic flow was 3.29×10⁸ m³ and the average basic flow index was 0.71 during 1954-2018. The basal flow showed a very significant decreasing trend in 1972 and 2000, and its periodic change was mainly controlled by the first main cycle (32 years). The variation of channel base flow in the blown-sand region area of the Wuding River Basin was the result of the comprehensive influence of precipitation, vegetation coverage, agricultural irrigation, etc. Compared with the first stage (1954-1972), in the second stage (1973-2000) and third stage (2001-2018) precipitation contributed 29.35% and 4.52% to the variation of baseflow, and the contribution rates of human activities (vegetation coverage and agricultural irrigation) were 70.65% and 95.48%, it indicates that human activities were the main driving factors of baseflow change. The research results can provide a basis for the rational development and utilization of water resources in blown-sand region of Wuding River Basin.