Abstract:Soil enzymes activities are important indicators for ecosystem function, playing key roles in nutrient cycling. In this study, five different land use types in Min River estuary wetland, i.e. reed wetland, tidal flat, grassland, farmland and fallow, were selected to investigate the effects of land use changes and restoration on soil enzymes activities as well as their relationship with soil properties. Soil enzymes activities including β—Glucosidase, N—Acetyl—β—glucosaminidase, L—Leucine aminopeptidase and phosphatase were detected. Results showed that soil pH, NH4+—N and NO3-—N contents were significantly higher in farmland than other land uses, while soil total carbon (C), nitrogen (N), and phosphorous (P) were significantly higher in fallow and reed wetland. Soil DOC, DON contents were significantly higher in fallow land, farmland and reed wetland than tidal flat and grassland. Soil microbial biomass carbon and nitrogen showed similar trend along different land uses with the highest in fallow land around 1 272 and 124 mg/kg, respectively. Soil enzymes activities showed similar pattern along different land uses, with the highest in fallow land, followed by reed wetland, farmland, grassland and tidal flat. Soil enzyme vector length ranged from 1.12~1.34, while all the vector angles were over 45°. Additionally, enzymatic eco-stoichiometric value was 1.00∶1.03∶1.33, indicating all the land uses were limited by phosphorus. Soil enzymes activities were significantly related with DOC, DON, microbial biomass C and N. These findings indicated that land use change from reed wetland to tidal flat significantly reduced soil nutrients, while fallow land increased soil microbial function, thereby providing alternative restoration strategy for wetland.