In order to find a feasible method to strengthen the phytoremediation of Cd contaminated soil, indoor culture experiments were conducted to study the effect of wood vinegar on the physical and chemical properties, enzyme activities, and Cd forms of calcareous heavy metal contaminated soils. Seven different dilution concentration treatments of wood vinegar were set up, and the soil physical and chemical properties, enzyme activities, Cd forms, and other indicators were measured and analyzed after adding wood vinegar and cultivation. The results showed that soil pH decreased gradually, and EC (except undiluted treatment) did not change significantly with increasing the concentration of wood vinegar. Comparing with CK, the content of soil organic matter, alkali hydrolyzable nitrogen, available phosphorus, and available potassium were increased by 0.35%~66.64%, 9.74%~96.10%, 31.71%~56.64% and 8.34%~30.87%. The soil enzyme activities (urease, invertase, and catalase activities) were increased significantly in 10-100 times dilution treatments, while soil enzyme activities were significantly inhibited in 5 times dilution and undiluted treatments (P<0.05). Except for the 5 times dilution treatment, the exchangeable Cd content in soil of other treatments was significantly higher than that of CK (P<0.05), increased by 24.69%~72.40%. Comparing with CK, the content of Cd in the carbonate-bound state, iron-manganese oxide state, and organic matter-bound state in all treatments were significantly increased by 6.67%~196.70%, 16.24%~80.09%, 31.51%~70.32%, and the residual Cd content was significantly reduced by 45.88~59.93% (P<0.05). In conclusion, adding appropriate concentration of wood vinegar to soil could increase soil nutrient content and enzyme activity, and promote the transformation of residual Cd in soil to other forms.