Taking sandy soil, fluvo-aquic soil and Shajiang soil in Dagu River Basin of Qingdao as research objects, the indoor soil column experiments was carried out to study the effects of soil temperature, pH, water content and other physical and chemical properties and combined application of biochar and nitrogen fertilizer on leaching of soil available phosphorus (Olsen-P) and total phosphorus (TP), so as to provide reference basis for improving soil phosphorus availability and reducing total phosphorus leaching. The results showed that the soil phosphorus availability was the highest when the pH was neutral or weakly alkaline, and the total phosphorus leaching loss was larger when the pH was weakly acidic. The Olsen-P content in different soil layers of the three soils showed different changes with the increasing of water content. The phosphorus availability of the three soils increased gradually with the increasing of temperature, while the leaching loss of total phosphorus decreased with the increasing of temperature. The combined application of biochar and nitrogen fertilizer (T1~T7) could effectively improve the availability of soil phosphorus, and the effect followed the order of combined application of biochar and nitrogen fertilizer> single addition of biochar> single addition of nitrogen. When the ratio of nitrogen fertilizer to biochar was 2:1, the availability of soil phosphorus was the highest. The inhibition effect of biochar combined with nitrogen fertilizer on soil total phosphorus leaching was better than that of biochar alone, and the inhibition effect of nitrogen fertilizer on soil total phosphorus leaching was not obvious.