Sedum plumbizincicola is a hyperaccumulator of Cd and Zn, and commonly used for the remediation of Cd-contaminated soil. Organic acids can increase the availability of heavy metals in soil, and enhance the accumulation of heavy metals in plants, and thus increase the plant remediation efficiency of heavy metal-contaminated and influence microbial quantity in soil. In this study, alluvial soil and reddish clay soil were used as test soils, and the effects of EDTA, citric acid, oxalic acid on remediation efficiency of Sedum plumbizincicola and soil microbial quantity were discussed. The results showed that organic acid could significantly increase the soil available Cd content, with the best effect given by citric acid. The available Cd contents in alluvial soil and reddish clay soil increased by 72.73% and 12.99% respectively compared to SCK (P<0.05). Sedum plumbizincicola in planted in two soils treated with EDTA had the highest shoot Cd content, which increased by 99.24% and 33.32% in the alluvial soil and reddish clay soil, respectively. The remediation efficiency of Sedum plumbizincicola cultivated in two soils treated with organic acid increased significantly compared with SCK. The application of organic acids increased significantly the quantity of microorganisms in soil compared with SCK, in which citric acid increased the quantity of bacteria and fungi in alluvial soil by 34.38% and 68.42% (P<0.05) respectively, and oxalic acid treatment increased the quantity of actinomycetes in reddish clay soil by 150.00%. This study could provide theoretical support for plant enhancement remediation technology used in heavy metal contaminated soils.