To investigate the alleviation of selenium (Se) on the chromium (Cr) toxicity in eggplant, the effects of different concentration of Se on the growth, physiological characteristics and Cr uptake by eggplant under different Cr stress levels were studied by pot experiments. The results showed that with the treatment of single Cr, the plant growth was significantly inhibited with the increase of Cr concentration. The Cr toxicity showed that the plant height, root length, shoot and dry weight and chlorophyll content were inhibited. With the increase of Cr concentrations, the chlorophyll and soluble protein in leaves showed a downward trend, and the content of malondialdehyde (MDA) increased, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) increased first and then decreased. The results of Cr-Se mixed treatment showed that Se could alleviate the Cr toxicity of eggplant. Under different concentrations of Cr stress, Se applied with different concentrations showed different alleviation effects. Studies showed that under the treatment of 10, 20, 40 and 80 mg/kg Cr, the good alleviation effects could be obtained when applied with 12, 24, 48 and 48 mg/kg Se respectively. The plant height, root length and biomass of eggplant increased, chlorophyll and soluble protein contents increased significantly, MDA and Cr accumulation in plant decreased.