In order to analyze the temporal stability characteristics of soil moisture content in Eucalyptus forests, nine sample points on a typical eucalyptus forest slope were selected to measure the soil moisture content for five years. Based on the measured data, the temporal and spatial variability and temporal stability of soil water content were analyzed by relative difference method and Spearman rank correlation coefficient method. The results illustrated that the average surface soil moisture content of five years of the study slope area was 21.81%, which showed weak variability in space and moderate variability in time. The correlation coefficient of average soil moisture content was low in each year, and the spatial pattern of soil water content had weak similarity in observation time, and the variation range of the average relative difference was -7.08% to 5.03%, and the average values of relative difference standard deviation and time stability index were 5.73% and 6.58%, respectively, indicating that the soil moisture had better time stability under different slope conditions. The moisture content of measuring points 2, 3, 6, 7 and 8 were higher than the average value, while the moisture content of measuring points 1, 4, 9, and 5 were relatively low. The standard deviation and time stability index of the wetting point were relatively small and stable, indicating that the moisture stability of soil in the wet state was better than that in the dry state. According to the regression analysis, there was a high correlation between the soil moisture content of point 5 and the mean water content of the whole slope, with the R² value of 0.879, the MBE value of 0.141 and the RMSE value of 99.4%. In other words, point 5 could be used as a representative measurement point to estimate the average soil moisture content in the shallow layer of the whole research area.