The effect of graphene solution combined with chemical fertilizer on the soil nutrients loss was studied by soil column leaching test, and the fertilizer retention effect of graphene solution was explored. A total of five graphene solution concentrations were designed, and four times leaching was carried out, the conductivity, nitrogen, phosphorus and potassium contents of each leaching solution and the soil after four times of leaching were determined. The results showed that:(1) Application of graphene solution combined with chemical fertilizer had effect on the conductivity, nitrogen, phosphorus and potassium contents of the leaching solution, and the greater the graphene solution concentration added, the more obvious the effect was. The content of nitrate nitrogen, total nitrogen and phosphorus in the leaching solution treated with graphene solution could be reduced by 88.2%, 80.9% and 84.7%, respectively. (2) After four times of leaching, the content of nitrogen, phosphorus, potassium and the conductivity value in the soil treated with graphene solution increased compared with the control, and the retention effects of total nitrogen and total phosphorus increased by 175.0% and 59.3%, but there was no significant effect on the retention of ammonium nitrogen. (3) The effect of graphene solution on the pH of the eluent and soil was not obvious. The application of graphene solution combined with chemical fertilizer into the soil could significantly reduce the leaching loss of soil nutrients, and had obvious retention effect of the soil nutrients. Graphene solution had the function of fertilizer conservation.