Potted Miaoxiang 7 strawberry was selected and ¹⁵N isotope tracer technology was used to investigate the effects of urea combined with 0, 2, 4 and 8 mL nano-carbon sol (CK, T1, T2 and, T3), on soil physiochemical properties, plant nitrogen uptake and utilization, and plant growth. The results showed that the application of nano-carbon significantly increased the soil redox potential and soil urease activity, and with the increasing of nano-carbon amount used, soil electrical conductivity showed a downward trend at the early stage and showed an increase trend at the later stage. The application of nano-carbon promoted the absorption and utilization of nitrogen in strawberry plants, increased Ndff value of each organ of strawberry. Compared with the CK, the nitrogen utilization efficiency of strawberry plants in T1, T2 and T3 increased by 71.2%, 126.8% and 98.9%, and the soil nitrogen residue rate increased by 8.2%, 16.7% and 16.1%, respectively, reduced nitrogen loss significantly. The application of nano-carbon increased the net photosynthetic rate, transpiration rate, stomatal conductance and the chlorophyll SPAD values of plant leaves in different degrees. Compared with the CK, dry matter of leaves in T1, T2 and T3 increased by 17.5%, 45.8%, and 32.3%, respectively. The study showed that the application of urea with nano-carbon could improve soil physical and chemical properties, adsorb nitrogen in soil effectively, increase plant nitrogen utilization rate and soil nitrogen residual rate, reduce nitrogen loss, and promote the growth of strawberry plants.