Abstract:In order to reveal the changing characteristics of soil organic carbon and nitrogen of light and heavy fractions in reclaimed soil as affected by different reclamation years and fertilization measures, the variation of carbon and nitrogen contents and C/N ratio of light and heavy fractions were studied under five fertilization modes by the density fractionation (no-fertilizer control, CK; single fertilizer, CF; single organic manure, M; fertilizer combined organic manure, MCF; and biological organic fertilizer and chemical fertilizer mixed fertilizer, MCFB). The results showed that TOC (total organic carbon), TON (total organic nitrogen), carbon and nitrogen of light fraction and heavy fraction all increased with the increasing ages. Single organic manure showed significant increase in TOC, TON, LFOC, HFOC, LFON and HFON compared with other treatments after 8 years of reclamation, and the corresponding contents were 148.10%,68.09%,163.68%,129.51%, 35.00% and 92.59% higher than those of raw soil, respectively. The sequence of C/N was Light Fraction C/N > Heavy Fraction C/N. The light fraction C/N in all treatments increased with the increasing reclamation ages, being the highest (25.48) in CF, and the heavy fraction C/N in all treatments decreased with increasing time, being the lowest (6.44) in CF. Fertilization measures had a significant effect on the management index of soil C and N stocks. Treatments of MCFB could improve CMI and NMI more effectively than other treatments. On the whole, under same nutrient inputs condition, single organic manure was more conducive to the accumulation of soil total organic carbon and nitrogen pools and carbon and nitrogen contents of light and heavy fractions in the reclaimed soil of coal mining subsidence areas. Single fertilizer promoted the increases of light fraction organic carbon and heavy fraction organic nitrogen to a greater extent.