In order to reveal the characteristics of nitrogen mineralization in reclaimed soil of coal mining subsidence area with different fertilization measures, this experiment used the method of intermittent leaching and aerobic culture to study the effects of five kinds of fertilization measures, such as no fertilizer application (CK), single application of chemical fertilizer (CF), single application of organic fertilizer (M), chemical fertilizer combined with organic fertilizer (MCF), and chemical fertilizer combined with biological organic fertilizer (MCFB) on the changes of mineral nitrogen and soluble organic nitrogen in reclamation soil for 4 years and 8 years, and the characteristics of nitrogen mineralization were also studied. The result showed that:As the years of reclamation increases, the accumulated NO₃^--N production in 8 years of reclamation is 2.63%~26.83% higher than that of 4 years, the cumulative production of NH₄⁺-N increases 12.50%~32.14%, the accumulative production of soluble organic nitrogen (SON) reduced by 31.59%~62.50% under CF and M treatments, and the other treatments increased 3.44%~34.69%. Under the same reclamation years, the accumulative production of NO₃^--N, NH₄⁺-N, and SON were the highest in MCFB. According to the mineralization parameters, the mineralization potential of the five fertilization measures was higher in 8 years than that in 4 years, and the increase range were MCFB(26.9%) > CK(15.9%) > CF(15.0%) > M(12.7%) > MCF(4.8%); Under CK and CF treatments, the potential mineralizable organic nitrogen (N₀/N) decreased with the increase of reclamation years, with the decrease rates being 9.4% and 13.8%, while the other three fertilization measures (N₀/N) showed an increasing trend, with the increase rate of MCF(13.2%)>MCFB(7.5%)>M(2.8%). Under MCF treatment, the mineralization rate (N_t/N) increased with the increase of reclamation years, and the increase rate was 13.2%, the mineralization rate of the other four fertilization measures was lower in 8 years than that in 4 years, and decreased by CF (12.1%) > CK (9.4%) > MCFB (7.5%) > M (2.7%). The values of N₀, N₀/N, and N_t/N in different treatments were the highest in 8 years of reclaimed soil with MCFB treatment. On the whole, after 8 consecutive years of reclamation, the biomineralization parameters of chemical fertilizer combined with biological organic fertilizer were improved to a greater extent than those of other fertilization measures. However, with the increase of reclamation years, the increase of N₀, N₀/N treated with chemical fertilizer and organic fertilizer was the largest, the reduction rate of N_t/N was the lowest in the treatment of chemical fertilizer combined with biological organic fertilizer, and the potential mineralizable organic nitrogen content decreased by (N₀/N) in long term single application of chemical fertilizer.