Pot experiments were carried out to evaluate the influence of Bacillus megaterum LY02 inoculation on phytoremediation of Cd, Cu and Cd-Cu contaminated soil by Lolium perenne L. The results showed that inoculation B. megaterum LY02 in Cd, Cu and Cd-Cu contaminated soil significantly increased the aboveground biomass of Lolium perenne L by 65%~108.3%. The strain promoted phytoextration of heavy metal by Lolium perenne L in different kinds of contaminated soil, and Cd extraction was increased significantly by 45.8% compared with the control. Compared with the control, B. megaterum L siginificantly enhanced the available phosphorus in three kinds of contaminated soil by 18.2%, 26.7% and 16.2%, respectively. The available Fe was increased after inoculation, especially in Cu-contaminated soil, the increase was up to 152.5%. Furthermore, the content of available Cd and Cu in the rhizosphere were also enhanced in the three kinds of contaminated soil, the available Cu was increased by 49.7% in the Cu-contaminated soil. Above all, inoculation B. megaterum LY02 in Lolium perenne L can improve the content of available P and Fe in the contaminated soil, and enhance the content of available Cd and Cu, which are beneficial for the improvement of remediation efficiency of Lolium perenne .