In order to improve the production of Trichosanthes kirilowii in the red soil and provide theoretical support for the cultivation of acid soils. Mainly in compare the difference of aluminum tolerance in between two places of origin, and determine the best relief concentration. Two kinds of Trichosanthes kirilowii Maxim., Baoding Trichosanthes kirilowii and Puyang Trichosanthes kirilowiiis selected as the experiment materials. Six different concentrations of Al3 + and abscisic acid were set up. To study the interaction of Al3+ and ABA on the root length, chlorophyll fluorescence characteristics, root activity, MDA content, antioxidant enzyme activity and antioxidant content of two species of the plant. The results show: (1) Baoding Trichosanthes kirilowii and Pu Yang Trichosanthes kirilowii under the effect of aluminum stress and exogenous abscisic acid, each index reflects similar trend, but varying degrees of each index and sensitivity revealed some differences, Baoding Trichosanthes kirilowii has stronger resistance ability of aluminum. (2)Aluminum threat forced two varieties of Trichosanthes kirilowii root length, root activity, Fv/Fm, Fm, ETR, SOD, POD, CAT and ASA, GSH content are decreased, and F0, MDA and DAsA rise. (3) After exogenous abscisic acid is added, two varieties of Trichosanthes kirilowii root length, root activity, chlorophyll fluorescence parameters except F0, anti-oxidase and ascorbate glutathione cycle system (ASA and GSH) show the trend of first increased and then decreased, MDA was decreased continuously, F0 and DAsA presented by the descending and ascending trend. 5 mg/L of exogenous abscisic acid make root length, root activity, Fv/Fm, Fm, ETR, SOD, POD, CAT and ASA, GSH rose to a peak, MDA decreased, F0 and DAsA down to the valley. (4)Through membership function and the analysis of the aluminum resistance coefficient, two varieties of Trichosanthes kirilowii can get the same result, the ability of Al tolerance: 5 mg/L＞7.5 mg/L＞2.5 mg/L＞0 mg/L＞10 mg/L. So the ability of aluminum resistance Baoding Trichosanthes kirilowii is stronger than Puyang Trichosanthes kirilowii, low concentration of exogenous abscisic acid can alleviate the stress of aluminum, but when the exogenous abscisic acid concentration is too high, unable to protect the plant from aluminum stress influence function, namely “promoting at the low concentration and high concentration inhibit”, 5 mg/L ABA can effectively relieve Al stress, So we should select aluminum resistant Trichosanthes kirilowii to plant, supplemented with appropriate concentration of exogenous ABA.