The arbuscular mycorrhiza fungi (AMF) was researched as an object in karst soil of different vegetation restoration stages (tree, bush and herb) of three typical karst sites (Huajiang, Zhijin and Huaxi), which were located in Guizhou Province, China. Some indexes such as species abundance, spore density, relative isolation frequency，important value，species evenness index and sample Shannon-Wiener diversity index were calculated after morphological identification, in order to explore the composition features and AMF species diversity in vegetation restoring process. The results showed as follows: The experimental soil totally contained 68 species of 10 genera, 8 families and 4 orders, and Glomus is the biggest genus by 35 species, and the second was Acaulospora (17 species), when Gigspora, Paraglomus and Archaeospora only have 1 species respectively in this study. Specially, Glomus lamellosum had biggest species abundance, relative isolation frequency and important value in all AMF species, which it was as an dominant species in three sampling sites. At the AMF species level, the abundance presented Tree＞Bush＞Herb in Huajiang,Bush＞Herb＞Tree in Zhijing, Bush＞Tree＞Herb in Huaxi. The evenness index and Shannon-wiener index of AMF species in different stages presented Tree＞Bush＞Herb in Huajiang and Zhijing sites, however the Shannon-wiener index in Huaxi site significantly higher than Tree and shrub stages. We suggested that the differences of heterogeneous conditions may affect on composition and diversity of AMF species.