The aim of this paper was to study the effect of phosphobacterias on phosphorus in a reclaimed soil. The tricalcium phosphate-solubilizing ability of individual phosphobacteria and their combinations was tested using laboratory shake-flask culture. The culture broth of Rahnella sp (W2) + Fluorescent pseudomonas1 (W3) + Fluorescent pseudomonas2 (W4) produced the highest available P content, 609.1 mg/L. Therefor, W2+W3+W4 was selected as the optimal combination of phosphobacterias. The effects of the optimal combination of phosphobacterias on soil nutrients, enzyme activities, P desorption properties, and maize yield were investigated by carrying out a field experiment in a reclaimed soil in a coal-mining subsidence area. The results showed that phosphobacteria increased soil nutrient contents and improved soil enzyme activities. Soil available P and K contents, as well as phosphatase, invertase, and urease activities, were increased 2.8, 25.43, 7.41, 4.21, and 7.15 mg/kg, respectively, by phosphobacteria compared to the medium control. The greatest reduction in the maximum P adsorption capacity (114 mg/kg) and adsorption constant (0.021) of soil was found with phosphobacterias compared to the blank control (p＜0.05). The mean P desorption rate of soil with phosphobacteria was 14.7, significantly higher than those in other treatments. The highest maize yield was also obtained with phosphobacteria, which was 8 036 kg/hm2 and significantly differed from those on other treatments. In conclusion, the application of phosphobacteria could improve soil P desorption properties, facilitate rapid improvement of soil fertility, and contribute to crop yield in reclaimed soil.