The aim of this study was to investigate the influence of phytoremediation on characterization of soil-contaminated by Mn mining tailings by using two species,Phytolacca americana and Glycine max.Pot culture experiments were conducted by using Mn mining tailings diluted with clean soil(80% Mn mining tailings 20% soils,DW).The soil organic matter,available N,P,and K increased after phytoremediation for 210 days.The soil pH did not change significantly.The activities of soil enzymes,such as phosphatase,urease,protease,sucrase,nitrate reductase,and dehydrogenase,were all higher in all treatments with phytoremediation than that in controls without phytoremediation.The distribution of Mn and Cd in each fraction were different between the substrates with and without phytoremediation treatments.Comparison with control treatment without phytoremediation,total Mn and Cd decreased in substrates with phytoremediation.The water soluble Mn and Cd were less in substrates with phytoremediation than that in controls.Phytoremediation efforts in areas contaminated by Mn mining tailings can be improved greatly by using mixtures including legume Glycine max and grass species Phytolacca americana.