The impact of human disturbances including grazing,cultivation and anti-grazing(from 1987,1997,2003 respectively) on nutrients contents(N,P,K and organic matter) in typical grassland soil was studied in the paper.The result showed that cultivation and grazing can lead to a decrease in nutrient contents of soil,especially topsoil,and cultivation affects it more significantly than grazing;Banned-grazing can increase nutrients contents of soil,which increase with banned-grazing period.At the same time,there exist significant minuslinear dependency relationship between the total N,available N,organic matter and soil depth;there exist second power function relation between available P,available K and soil depth.The total P contents increases with the soil depth,and the total K decreases with it,but the dependency relations were not significant.It is concluded that anti-grazing can increase the nutrient contents in grassland soil and restrain the degradation of grassland soil.