Application of bacteria, which were named as B2 and B67 isolated by the authors, could enhance crop yield and quality. The content of available phosphate and fertility of soil were improved, especially along with chemical fertilizer. Height, fresh weight and dry weight of maize were increased to 15.2 % - 89.2 % respectively, by combination of phosphate liberation bacteria with chemical fertilizer relatively to control. The nutrient accumulation per plant was increasedto 20 % - 146% compared with control. The fresh weight, dry weight and biomass of Chinese cabbage were increased to 15.8%-41.6% by phosphate liberation bacteria with chemical fertilizer compared with those of control. Height, grain number per ear, grain weight per ear and biomass of wheat were significant enhanced by combination of phosphate liberation bacteria with chemical fertilizer. Field experiment demonstrated that combination of phosphate liberation bacteria with chemical fertilizer promoted yield production for a few crops. When bacteria B2 and B67 were incubated to Shanxi typical soil types, available phosphate was enhanced and was roughly 1.35-3.04 times of that in control soil sample. Also it was found that phosphate lyolysis was positively related to available number of bacteria and phosphatase activity in soil. Phosphate liberation bacteria improved both available phosphate and available potassium in the soil.