The effects of spraying plant growth regulator under drought stress on seedling growth and physiological and biochemical indexes of soybean were studied based on pot experiment. The results indicated that the plant growth regulator could increase plant height, main root length, lateral root number, root/shoot ratio, chlorophyll content, proline content and the activity of peroxydase(POD), and improve the ability of drought-resistance of soybean seedlings. Among the 4 plant growth regulators used in this experiment, PSB was the best effective for the drought-resistance of soybean seedlings.