The Three Gorges and upstream areas is the most severe soil erosion areas in China. The concentration of NH_3-N,NO_2-N,NO_3-N,TN and TP were determined every month during June to December in 2003.Six horizontal monitoring sites and two vertical sites were set in Three Gorges reservoir. The results showed that the six surface layer water mean concentration of NH_3-N,NO_2-N,NO_3-N,TN,TP were 0.11 mg/L,0.019 mg/L,1.28 mg/L,1.62 mg/L,0.13 mg/L respectively. Along the flow direction, the concentration of TP were depressed distinctly and TN were increased. There were not the trend on NO_2-N,NO_3-N and NH_3-N.According to different depth (surface,0.6depth and 0.8depth),three monitoring sites were set in the direction of vertical. Through successive seven months monitoring, it indicated concentration of nutrient were not variety distinctly. Changing of phosphorus concentration was related to flow of the river. The maximum of total phosphorus was appeared in high flow season ,and the minimum was appeared in low flow season.