On the basis of 25-year study on long-term located experiments of fertilizer,analysis is made on he change tendency of heavy-metal content in soil of 0~20 cm which are treatd with six different fertilization trails detailed as fertilizing by N,NP,NPK,N organic fertilizer,N straw and no fertilizer. The result states that: All 6 elements present trend of increasing. The Zn content of the soil with N organic fertilizer processed, which exceeds the limit of the Soil Environmental Quality Standard II, Hg content increased massively, 114% increased than the testing foundational value. No weighty effect to the soil only N fertilizer (urea) used, use P fertilizer shall increase the heavy-metal content of the soil. The organic fertilizer, which takes rejected materials of livestock and poultry as raw materials, shall increase the heavy-metal content of the soil, Cu, Zn and Hg increased especially. The organic fertilizer, which takes rejected materials of livestock and poultry as raw materials, should be used reasonably, fertilization is an important reason to effect the change of heavy-metal in soil.