Distribution of soil microbial physiological community and intensity of soil biological activity among different karst landscape in the Guilin Yaji Karst Experimental Site were studied. The results showed that variation of the intensity of soil biological activity was almost in accordance with changes in soil microbial number. The relationship between soil microbial number and organic matter, total N, available N, and available P of soil samples, and the ecological effect of soil microbial physiological community participating nitrogen cycle in this area were also discussed.